Human Tyro3/Dtk Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF859
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tyro3/Dtk
Ala41-Ser428
Accession # Q06418
Ala41-Ser428
Accession # Q06418
Specificity
Detects human Tyro3/Dtk in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse Tyro3/Dtk, recombinant human (rh) Axl, and rhMer is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Tyro3/Dtk Biotinylated Antibody
Human Tyro3 / Dtk ELISA Standard Curve
Recombinant Human Tyro3/Dtk Fc Chimera (Catalog # 859-DK) was serially diluted and captured by Mouse Anti-Human Tyro3/Dtk Monoclonal Antibody (Catalog # MAB859) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF859) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Tyro3/Dtk Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Tyro3/Dtk Fc Chimera (Catalog # 859-DK)
Sample: Recombinant Human Tyro3/Dtk Fc Chimera (Catalog # 859-DK)
Human Tyro3/Dtk Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tyro3/Dtk
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.
References
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Alternate Names
Brt, Dtk, Rse, Sky
Gene Symbol
TYRO3
UniProt
Additional Tyro3/Dtk Products
Product Documents for Human Tyro3/Dtk Biotinylated Antibody
Product Specific Notices for Human Tyro3/Dtk Biotinylated Antibody
For research use only
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