Human TWEAK/TNFSF12 Antibody

Name: Human TWEAK/TNFSF12 Antibody
Brand: R&D Systems
SKU: AF1090
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1090

Citations (3)

(1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human TWEAK Arg93-His249 Accession # Q4ACW9
Applications:	Block/Neutralize, Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human TWEAK in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) BAFF, rhFas Ligand, rhGITR Ligand, rhOX40 Ligand, rhTRAIL and less than 1% cross-reactivity with rhAPRIL, rhLIGHT, rhTNF-alpha, rhVEGI, and rhTRANCE is observed.

View Full Technical Details for Human TWEAK/TNFSF12 Antibody

Applications for Human TWEAK/TNFSF12 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW)
Immunocytochemistry	5-15 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize TWEAK/TNFSF12-induced inhibition of proliferation in the HT‑29 human colon adenocarcinoma cell line. Yu, K. Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J. A. et al. (1998) J. Biol. Chem. 273:27548The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 1 µg/mL Recombinant Human TWEAK/TNFSF12.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human TWEAK/TNFSF12 Antibody

	Neutralization TWEAK/TNFSF12 Inhibition of Cell Proliferation and Neutralization by Human TWEAK/TNFSF12 Antibody. Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human TWEAK/TNFSF12 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090). The ND₅₀ is typically 2-8 µg/mL.
	Immunocytochemistry TWEAK/TNFSF12 in MDA-MB-231 Human Cell Line. TWEAK/TNFSF12 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Goat Anti-Human TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (amino acids (aa) 1-18), the transmembrane domain (aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1, or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).

Item	Value
References	Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401. Marsters, S. et al. (1998) Current Biol. 8:525. Wiley, S.R. et al. (2001) Immunity, 15:837. Feng, S-L.Y. et al. (2000) Am J. Path. 156:1253. Nakayama, M. et al. (2002) J. Immunol. 168:734. Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Long Name	TNF-like and Weak Inducer of Apoptosis
Entrez Gene IDs	8742 (Human); 21944 (Mouse); 360548 (Rat)
Alternate Names	APO3 ligand, APO3LMGC20669, DR3LGTWEAKAPO3/DR3 ligand, MGC129581, TNF-related weak inducer of apoptosis, TNFSF12, TWEAK, tumor necrosis factor (ligand) superfamily, member 12, tumor necrosis factor ligand superfamily member 12

Citations for Human TWEAK/TNFSF12 Antibody (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human TWEAK/TNFSF12 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Stephan D et al. (2013), TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier. J Neuroinflammation, 10(0):9.
PMID: 23320797
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Inoue T et al. (2011), Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins. Int. J. Oncol., 38(5):1299-306.
PMID: 21373756
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

Moreno JA et al. (2010), Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio. Arterioscler. Thromb. Vasc. Biol., 30(6):1253-62.
PMID: 20299688

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Customer Reviews for Human TWEAK/TNFSF12 Antibody (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Human TWEAK/TNFSF12 Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 11/08/2019

This antibody was used as the detection in an ELISA with MAB1090 as the capture.

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Product Documents for Human TWEAK/TNFSF12 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human TWEAK/TNFSF12 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human TWEAK/TNFSF12 Antibody

Scientific Data Examples for Human TWEAK/TNFSF12 Antibody

Neutralization

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: TWEAK/TNFSF12

Citations for Human TWEAK/TNFSF12 Antibody (3)

Customer Reviews for Human TWEAK/TNFSF12 Antibody (1)

Customer Images

Human TWEAK/TNFSF12 Antibody

Product Documents for Human TWEAK/TNFSF12 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected