Human TROY/TNFRSF19 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1548S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TROY/TNFRSF19
Glu30-Leu170
Accession # AAF71828
Glu30-Leu170
Accession # AAF71828
Specificity
Detects human TROY/TNFRSF19 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) TROY is observed and less than 1% cross-reactivity with recombinant human (rh) 4-1BB, rhBA
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human TROY/TNFRSF19 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: TROY/TNFRSF19
TROY, also known as TAJ (Toxicity and JNK inducer), and TRADE, is a type I tramsmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19. Two alternatively spliced transcript variants for human TROY, encoding a 423 amino acid (aa) residues isoforms 1 precursor and a 417 aa isoform 2 precursor have been cloned. The two isoforms differ in their cytoplasmic C-terminal residues. In mouse, a soluble TROY isoform and a transmembrane isoform with a short cytoplasmic tail have also been described. Human TROY isoform 1 cDNA encodes a precursor protein with a putative 23 aa signal peptide, a 147 aa extracellular domain containing two TNF receptor cysteine-rich domains, a 23 aa transmembrane domain and a 230 aa cytoplasmic region containing no death domain. The extracellular domain of human TROY shares 92% aa sequence identity with that of the mouse TROY. In their cytoplasmic domains, the two proteins also share 57% sequence homology. Among TNFRSF members, the extracellular region of TROY is most closely related to EDAR, exhibiting 33% homology. TROY expression has been found in many different embryonic and adult tissues, and is particularly strong in embryonic brain and skin. TROY has been shown to interact with TRAF family members, and to activate the JNK signaling pathway. Although TROY lacks a death domain, it can induce apoptosis by a caspase-independent mechanism. It has been suggested that TROY has pleiotropic functions during embryonic development as well as in adults, particularly in the development of skin and hair follicles. The ligand for TROY has not been identified (1‑3).
Long Name
Tumor Necrosis Factor Receptor Superfamily, Member 19
Alternate Names
TAJ, TNFRSF19
Gene Symbol
TNFRSF19
UniProt
Additional TROY/TNFRSF19 Products
Product Specific Notices for Human TROY/TNFRSF19 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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