Human Transcobalamin II Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7895R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Transcobalamin II
Glu19-Trp427
Accession # P20062
Glu19-Trp427
Accession # P20062
Specificity
Detects human Transcobalamin II in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Transcobalamin II
TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. It is a key player in the transport and absorption of VitB12. TCN1 is a salivary gland protein that binds oral/dietary B12. Once in the stomach, B12 is transferred to IF/Intrinsic Factor, and this complex remains intact during passage through the small intestine. In the distal ileum, the IF:VitB12 complex is internalized following binding to cubam on enterocyte membranes. Once internalized, VitB12 is processed and re-expressed on MRP1 on the enterocyte's basal surface. Here it is transferred to TCN-2, a binding protein secreted by endothelial cells that accounts for the transport of anywhere from 10-30% of circulating VitB12/cobalamin. The TCN2:VitB12 complex is ultimately internalized by cells expressing a membrane-bound, 58 kDa TCN2 receptor termed TCblR. Once internalized, VitB12 is released and converted into cofactors that are involved in methionine biosynthesis. Human TCN2 is synthesized as a 427 amino acid (aa) precursor that contains an 18 aa signal sequence, plus a 409 aa mature region that binds one VitB12 molecule. There are three potential splice variants of TCN2. One contains a Trp substitution for aa 116-143, a second possesses an Ile substitution for aa 336-427, and a third shows a deletion of aa 143-193. There is one Arg-to-Pro transition at position 259 that is correlated with statistically elevated circulating levels of TCN2. Over aa 19-427, human TCN2 shares 72% aa sequence identity with mouse TCN2.
Alternate Names
TC-2, TCII, TCN2, Vitamin B12-binding Protein 2
Gene Symbol
TCN2
UniProt
Additional Transcobalamin II Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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