Human TRANCE/TNFSF11/RANK L Antibody
Catalog # AF626 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Customers Also Viewed
Product Summary for Human TRANCE/TNFSF11/RANK L Antibody
Immunogen
Gln73-Asp317 (Ala194Gly)
Accession # O14788
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human TRANCE/TNFSF11/RANK L Antibody
Osteoclast-Like Cell Form-ation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody.
In the presence of Recombinant Mouse M-CSF (20 ng/mL, 416-ML), Recombinant Human TRANCE/ TNFSF11/RANK L (390-TN) induces osteoclast-like cell formation in RAW 264.7 mouse macrophages in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/TNFSF11/ RANK L (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human TRANCE/TNFSF11/ RANK L Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF626). The ND50 is typically 0.01-0.04 µg/mL.Applications for Human TRANCE/TNFSF11/RANK L Antibody
Western Blot
Sample: Recombinant Human TRANCE/TNFSF11/RANK L (Truncated) (Catalog # 390-TN)
Neutralization
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRANCE/TNFSF11/RANK L
RANK Ligand (receptor activator of NF-kappa B ligand [RANKL], also called TNF-related activation-induced cytokines (TRANCE), osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. RANK Ligand was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The human RANK Ligand cDNA encodes a type II transmembrane protein of 317 amino acids with a predicted cytoplasmic domain of 47 amino acids, a 21 amino acids transmembrane region, and an extracellular domain of 249 amino acids. The extracellular domain contains two potential N‑linked glycosylation sites. Mouse and human RANK Ligand share 85% amino acid identity. RANK Ligand is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of RANK Ligand include induction of activation of the c-jun N‑terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of RANK Ligand. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds RANK Ligand, is a naturally occurring decoy receptor that counterbalances the effects of RANK Ligand.
References
- Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
- Anderson, D.M. et al. (1997) Nature 390:175.
- Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
- Kong, Y-Y. et al. (1999) Nature 397:315.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Human TRANCE/TNFSF11/RANK L Antibody
Product Specific Notices for Human TRANCE/TNFSF11/RANK L Antibody
For research use only
Citations for Human TRANCE/TNFSF11/RANK L Antibody (3)
Citations are publications that use Bio-Techne products. Selected citations for Human TRANCE/TNFSF11/RANK L Antibody include:
-
Sample Types: Protein
Applications: Binding AssayJustin R Savage (2016-06-16), A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis PLoS ONE, 2016-011(6):e0157310.
PMID: 27308827 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotBuckle et al. (2012-08-29), Soluble rank ligand produced by myeloma cells causes generalised bone loss in multiple myeloma. PLoS ONE, 2012-07(8):e41127.
PMID: 22952578 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PGrimaud E et al. (2003-11-01), Receptor activator of nuclear factor kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio is increased in severe osteolysis. Am. J. Pathol., 2003-1163(5):2021-31.
PMID: 14578201
There are no citations that match your criteria.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.