Human TRAILR1/TNFRSF10A Biotinylated Antibody

Name: Human TRAILR1/TNFRSF10A Biotinylated Antibody
Brand: R&D Systems
SKU: BAF347

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF347

Citations (4)

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Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRAIL R1/TNFRSF10A Ala24-Asn239 Accession # AAC51226
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human TRAIL R1/TNFRSF10A in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) TRAIL R2 is observed and no cross-reactivity with rhTRAIL R3 and rhTRAIL R4 is observed.

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Applications for Human TRAILR1/TNFRSF10A Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human TRAIL R1/TNFRSF10A Fc Chimera (Catalog # 347-DR)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TRAILR1/TNFRSF10A

Human TRAIL R1, also called DR4, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL RI is referred to as TNFRSF10A. TRAIL R1 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among the TNF receptor family proteins, TRAIL R1 is most closely related to TRAIL R2/DR5, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R1/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R1, an additional TRAIL R2/DR5, which tranduces apoptosis signal, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

Item	Value
References	Pan, G. et al. (1997) Science 276:111. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name	TRAIL Receptor 1
Entrez Gene IDs	8797 (Human)
Alternate Names	APO2, CD261, CD261 antigen, DR4, DR4 TRAIL receptor 1, Death receptor 4, TNF-related apoptosis inducing ligand receptor 1, TNF-related apoptosis-inducing ligand receptor 1, TNFRSF10A, TRAIL R1, TRAIL-R1, TRAILR-1, TRAILR1, TRAILR1MGC9365, cytotoxic TRAIL receptor, tumor necrosis factor receptor superfamily member 10A, tumor necrosis factor receptor superfamily, member 10a

Citations for Human TRAILR1/TNFRSF10A Biotinylated Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human TRAILR1/TNFRSF10A Biotinylated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Gatti L et al. (2014), Improved apoptotic cell death in drug-resistant non-small-cell lung cancer cells by tumor necrosis factor-related apoptosis-inducing ligand-based treatment. J Pharmacol Exp Ther, 348(3):360-71.
PMID: 24345465
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Reddy RM et al. (2007), Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade. Apoptosis, 12(1):55-71.
PMID: 17136498
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Tsai WS et al. (2006), Enhancement of Apo2L/TRAIL-mediated cytotoxicity in esophageal cancer cells by cisplatin. Mol. Cancer Ther., 5(12):2977-90.
PMID: 17172403
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Mouzakiti A et al. (2003), Regulation of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Burkitt's lymphoma cell lines. Br. J. Haematol., 122(1):61-9.
PMID: 12823346

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Showing 1 - 4 of 4 citations Showing All

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Human TRAILR1/TNFRSF10A Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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