Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

Name: Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
Brand: R&D Systems
SKU: MAB4770
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB4770

Citations (9)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	2102Ep human embryonal carcinoma cell line
Applications:	CyTOF-reported, Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgM Clone # TRA-1-60
Specificity:	Detects human TRA‑1‑60(R).

View Full Technical Details for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

Applications for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-reported	This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

	Flow Cytometry Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).
	Immunocytochemistry TRA‑1‑60 in BG01V Human Stem Cells. TRA-1-60 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (yellow; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunocytochemistry TRA‑1‑60(R) in ADLF1 and FAB2 Stem Cell Lines. TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA-1-60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Formulation, Preparation and Storage

Purification	IgM-specific Affinity-purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

Item	Value
References	Andrews, P. et al. (1984) Hybridoma 3:347. Thomson, J. et al. (1998) Science 282:1145. Giwercman, A. et al. (1993) Cancer 72:1308. Marrink, J. et al. (1991) Int. J. Cancer 49:368. Badcock, G. et al. (1999) Cancer Res. 59:4715. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name	Tumor-related Antigen-1-60 [R]
Entrez Gene IDs	5420 (Human)
Alternate Names	RA160, TRA-1-60(R), TRA160(R), tra61

Citations for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody (9)

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Citations are publications that use Bio-Techne products. Selected citations for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Y Nagai et al. (2021), Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies Biomolecules, 11(4).
PMID: 33805466
Species: Human
Sample Types: Whole Cells
Applications: ICC

SJ Walker et al. (2021), A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines Heliyon, 7(4):e06617.
PMID: 33869861
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Luo et al. (2021), Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development Stem Cell Research, 57(0):102583.
PMID: 34710837
Species: Human
Sample Types: Whole Cells
Applications: ICC

DJ Sutcliffe et al. (2021), Induced pluripotent stem cells from subjects with Lesch-Nyhan disease Scientific Reports, 11(1):8523.
PMID: 33875724
Species: Human
Sample Types: Whole Cells
Applications: Labeling

MS Breen et al. (2020), Transcriptional signatures of participant-derived neural progenitor cells and neurons implicate altered Wnt signaling in Phelan-McDermid syndrome and autism Mol Autism, 11(1):53.
PMID: 32560742
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

T Yamamoto et al. (2019), Generation of three induced pluripotent stem cell lines from postmortem tissue derived following sudden death of a young patient with STXBP1 mutation Stem Cell Res, 39(0):101485.
PMID: 31255830
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

PA De Sousa et al. (2017), Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience Stem Cell Res, 20(0):105-114.
PMID: 28334554
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells. BMC Vet Res, 8(0):150.
PMID: 22937862
Species: Human
Sample Types: Whole Cells
Applications: ICC

Kim SY et al. (2012), Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells. Stem Cells Dev., 21(8):1272-86.
PMID: 21787230

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Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

Name: Anonymous

Application: Flow

Sample Tested: H9 human embryonic stem cells

Species: Human

Verified Customer | Posted 10/20/2017

The cells were stained with 1 µg of TRA-1-60 antibody in 100 µl FACS buffer at 4C for 30 min. After washing, the cells were incubated with anti-IgM-AF488 antibody at 4C for 20 min.

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Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

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Applications for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

Scientific Data Examples for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

Flow Cytometry

Immunocytochemistry

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: TRA-1-60(R)

Citations for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody (9)

Customer Reviews for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody (1)

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Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody

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