Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-reported

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-60

View all TRA-1-60(R) Antibodies »

Product Specifications

Immunogen

2102Ep human embryonal carcinoma cell line

Specificity

Detects human TRA‑1‑60(R).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Applications

Application
Recommended Usage

CyTOF-reported

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

References

  1. Andrews, P. et al. (1984) Hybridoma 3:347.
  2. Thomson, J. et al. (1998) Science 282:1145.
  3. Giwercman, A. et al. (1993) Cancer 72:1308.
  4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
  5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
  6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.

Long Name

Tumor-related Antigen-1-60 [R]

Alternate Names

TRA160(R)

Entrez Gene IDs

5420 (Human)

Gene Symbol

PODXL

Additional TRA-1-60(R) Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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