Human TOMM20 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11604
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1074702
Product Specifications
Immunogen
TOMM20 containing synthetic peptide
Accession # Q15388
Accession # Q15388
Specificity
Detects a synthetic peptide specific for human TOMM20 containing amino acid 55 in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TOMM20 Antibody
Detection of TOMM20 in A549 Human Cell Line.
TOMM20 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the mitochondrion membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human TOMM20 by Western Blot.
Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TOMM20 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of TOMM20 in Human Lung.
TOMM20 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of bronchial epithelium. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human TOMM20 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung and testis
Sample: Immersion fixed paraffin-embedded sections of human lung and testis
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TOMM20
References
- Park SH, Lee AR, Choi K, Joung S, Yoon JB, Kim S. TOMM20 as a potential therapeutic target of colorectal cancer. BMB Rep. 2019 Dec;52(12):712-717. doi: 10.5483/BMBRep.2019.52.12.249. PMID: 31818360; PMCID: PMC6941759.Nijenjuis M., Hämmerling GJ. J Immunol. 1996 Dec 15;157(12):5467-77.
- Yin L, Ye Y, Zou L, Lin J, Dai Y, Fu Y, Liu Y, Peng Y, Gao Y, Fu Y, Qi X, Deng T, Zhang S, Li X. AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. J Exp Clin Cancer Res. 2023 Aug 10;42(1):204. doi: 10.1186/s13046-023-02776-0. PMID: 37563661; PMCID: PMC10413764.
Long Name
TOMM20
Alternate Names
MAS20, mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolo, MOM19, outer mitochondrial membrane receptor Tom20, TOM20, translocase of outer mitochondrial membrane 20 hom
Gene Symbol
Q15388
UniProt
Additional TOMM20 Products
Product Documents for Human TOMM20 Antibody
Product Specific Notices for Human TOMM20 Antibody
For research use only
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