Human TLR7 PerCP-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC5875C

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IC5875C
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

PerCP (Excitation = 482 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 533707

View all TLR7 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TLR7
Met360-Leu516
Accession # Q9NYK1

Specificity

Detects human TLR7 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TLR7 PerCP-conjugated Antibody

Detection of TLR7 antibody in Ramos Human Cell Line antibody by Flow Cytometry.

Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry.

Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PerCP-conjugated Monoclonal Antibody (Catalog # IC5875C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human TLR7 PerCP-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Ramos human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR7

Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.

Long Name

Toll-like Receptor 7

Alternate Names

toll-like receptor 7

Entrez Gene IDs

51284 (Human); 170743 (Mouse); 317468 (Rat)

Gene Symbol

TLR7

UniProt

Q9NYK1

Additional TLR7 Products

Product Documents for Human TLR7 PerCP-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human TLR7 PerCP-conjugated Antibody

For research use only

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