Human TIMP-3 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC9731A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 277128
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-3
Cys24-Pro211
Accession # P35625
Cys24-Pro211
Accession # P35625
Specificity
Detects human TIMP-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TIMP-1, -2, or -4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human TIMP-3 APC-conjugated Antibody
Detection of TIMP‑3 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TIMP-3 APC-conjugated Monoclonal Antibody (Catalog # IC9731A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Intracellular Molecules.Applications for Human TIMP-3 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line
Sample: MDA‑MB‑231 human breast cancer cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: TIMP-3
Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE.
TIMP-3 is unique among the TIMPs because of its high affinity for binding to the extracellular matrix. Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.
Long Name
Tissue Inhibitors of Metalloproteinases 3
Alternate Names
TIMP3
Gene Symbol
TIMP3
UniProt
Additional TIMP-3 Products
Product Documents for Human TIMP-3 APC-conjugated Antibody
Product Specific Notices for Human TIMP-3 APC-conjugated Antibody
For research use only
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