Human TIMP-3 Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF973AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects human TIMP-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TIMP‑1, rhTIMP‑2, and rhTIMP‑4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human TIMP-3 Alexa Fluor™ Plus 680-conjugated Antibody
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: TIMP-3
Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE (1). TIMP-3 is unique among the TIMPs because of its high affinity for binding to the extracellular matrix (2). Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.
References
- Amour, A. et al. (1998) FEBS Lett. 435:39.
- Leco, K.J. et al. (1994) J. Biol. Chem. 269:9352.
Long Name
Tissue Inhibitors of Metalloproteinases 3
Alternate Names
TIMP3
Gene Symbol
TIMP3
UniProt
Additional TIMP-3 Products
Product Specific Notices for Human TIMP-3 Alexa Fluor™ Plus 680-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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