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Human TFF3 Antibody

Bio-Techne includes R&D Systems | Catalog # AF4407

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AF4407
AF4407-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Summary for Human TFF3 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654

Specificity

Detects human Trefoil Factor 3 (TFF3) in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TFF3 Antibody

Detection of Human TFF3 antibody by Western Blot.

Detection of Human TFF3 by Western Blot.

Western blot shows lysates of human pancreas tissue and KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TFF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TFF3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TFF3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human pancreas tissue and KATO‑III human gastric carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF4407 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF3

TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease-resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. Mature human TFF3 is 59 amino acids (aa) in length (aa 22-80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond-mediated loops (aa 30-72). Human TFF3 exists as both a monomer and disulfide-linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N‑terminal extension and a third shows a 109 aa substitution for aa 29-80. Over aa 21-80, human TFF3 shares 77%, 68%, and 72% aa identity with mouse, canine, and rat TFF3, respectively.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Entrez Gene IDs

7033 (Human); 21786 (Mouse); 25563 (Rat)

Gene Symbol

TFF3

UniProt

Additional TFF3 Products

Product Documents for Human TFF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TFF3 Antibody

For research use only

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