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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human TCF7/TCF1
Met116-His233
Accession # P36402

Specificity

Detects human TCF7/TCF1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human TCF7/TCF1 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TCF7/TCF1

TCF7 (Transcription factor 7; also T cell factor 1/TCF1) is a 25‑50 kDa member of the lymphoid enhancer binding factor family of proteins with 16 isoforms. It is expressed in thymocytes and mature T cells, and serves multiple purposes. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, large TCF7 isoforms predominate (42‑50 kDa), and serve a transcriptional activator function. Human TCF7 is 384 amino acids (aa) in length. This is likely an activating isoform that contains a beta-catenin binding domain (aa 1‑59), a DNA-binding HMG-box (aa 269‑337), and an NLS (aa 344‑348). The use of an alternate start site at Met116 seems to characterize repressor isoforms.

Long Name

Transcription Factor 7

Alternate Names

TCF1

Entrez Gene IDs

6932 (Human); 21414 (Mouse); 363595 (Rat)

Gene Symbol

TCF7

UniProt

Additional TCF7/TCF1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TCF7/TCF1 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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