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Human Syndecan‑4 Antibody

Bio-Techne includes R&D Systems | Catalog # AF2918

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AF2918
AF2918-SP

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Syndecan‑4 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-4
Glu19-Glu145
Accession # P31431

Specificity

Detects human Syndecan-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Syndecan-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Syndecan‑4 Antibody

Syndecan-4 antibody in HeLa Human Cervical Epithelial Carcinoma Cell Line by Immunocytochemistry (ICC).

Syndecan‑4 in HeLa Human Cervical Epithelial Carcinoma Cell Line.

Syndecan-4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Syndecan-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Syndecan‑4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Syndecan‑4 (Catalog # 2918-SD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using AF2918 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-4

Syndecan-4, previously known as amphiglycan or ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

References

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488. 
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. David, G. et al. (1992) J. Cell Biol. 118:961. 
  4. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 
  5. Charnaux, N. et al. (2005) FEBS J. 272:1937.  
  6. Schmidt, A. et al., J. Biol. Chem. 280:34441.
  7. Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
  8. Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
  9. Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
  10. Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.

Alternate Names

Amphiglycan, Ryudocan, SDC4, Syndecan4

Entrez Gene IDs

6385 (Human); 20971 (Mouse)

Gene Symbol

SDC4

UniProt

Additional Syndecan-4 Products

Product Documents for Human Syndecan‑4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Syndecan‑4 Antibody

For research use only

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FAQs for Human Syndecan‑4 Antibody

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  • Q: If Human Syndecan-4 is found on the membrane surface, and the immunogen is the extracellular portion of the protein, why does the ICC example for Catalog # AF2918 appear to have cytoplasmic staining?

    A: Our ICC protocol includes a permeabilization step prior to staining with the antibody, so the antibody will stain Syndecan-4 if present in the cytoplasm in addition to Syndecan-4 on the cell surface. 

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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