Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Human Syndecan-2/CD362 APC‑conjugated Antibody

Bio-Techne includes R&D Systems | Catalog # FAB2965A

Clone 305515 was used by HLDA to establish CD designation

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 305515

Product Summary for Human Syndecan-2/CD362 APC‑conjugated Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-2
Glu19-Gly144
Accession # AAH49836.1

Specificity

Detects human Syndecan-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Syndecan-1, recombinant human (rh) Syndecan-3, or rhSyndecan-4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Syndecan-2/CD362 APC‑conjugated Antibody

Detection of Syndecan-2/CD362 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of Syndecan-2/CD362 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Rat Anti-Human Syndecan-2/CD362 APC-conjugated Monoclonal Antibody (Catalog # FAB2965A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Detection of Syndecan-2/CD362 antibody in Colo205 cells antibody by Flow Cytometry.

Detection of Syndecan-2/CD362 in Colo205 cells by Flow Cytometry.

COLO 205 human colorectal adenocarcinoma cell line was stained with Rat Anti-Human Syndecan-2/CD362 APC-conjugated Monoclonal Antibody (Catalog # FAB2965A, filled histogram) or Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Syndecan-2/CD362 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes or COLO 205 human colorectal adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 10 publications using FAB2965A in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Syndecan-2/CD362

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2 is dependent on the transmembrane domain (5). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines (6). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGF basic, VEGF and EGF (7). Syndecan-2 expressed on human primary osteoblasts binds GM-CSF and may function as a co‑receptor (8). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (9). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (10).

References

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.
  4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.
  5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
  6. Park, H. et al. (2002) J. Biol. Chem. 277:29730.
  7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.
  8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.
  9. Halden, Y. et al. (2004) Biochem. J. 377:533.
  10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.

Alternate Names

CD362, Fibroglycan, HSPG1, SDC2, SYND2, Syndecan2

Entrez Gene IDs

6383 (Human); 15529 (Mouse)

Gene Symbol

SDC2

UniProt

Additional Syndecan-2/CD362 Products

Product Documents for Human Syndecan-2/CD362 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Syndecan-2/CD362 APC‑conjugated Antibody

For research use only

Loading...