Human STAT2 Antibody

Detection of STAT2 by Western Blot

IFN-beta and IFN-lambda mediate STAT1 and STAT2 activation upon RSV and IAV infection in A549 cells. (A and B) ELISA for IFN-beta following RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Open circles mark data points with cytokine concentrations below the detection range. Error bars are standard deviations (SD) from technical replicates. (C) IFN-beta (green) (left) and activation of STAT1 (yellow) (right) and IRF3 (red) 24 h after infection of A549 WT cells with RSV (magenta, polyclonal anti-RSV antibody) at an MOI of 0.01. White dotted lines are nuclear outlines determined based on DAPI counterstaining (channel not shown). Bars, 50 μm. (D and E) Activation of STAT1, STAT2, and interferon-stimulated genes (RIG-I, PKR, and OAS1) upon RSV (D) and IAV infection (E) at an MOI of 0.1 at 24 and 48 h postinfection (p.i.) in A549 WT, IFNAR1 KO, IFNLR1 KO, and IFNAR1-IFNLR1 dKO cells. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. RSV F, RSV fusion glycoprotein; IAV NP, IAV nucleoprotein. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of STAT2 by Western Blot

IFN-beta and IFN-lambda mediate STAT1 and STAT2 activation upon RSV and IAV infection in A549 cells. (A and B) ELISA for IFN-beta following RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Open circles mark data points with cytokine concentrations below the detection range. Error bars are standard deviations (SD) from technical replicates. (C) IFN-beta (green) (left) and activation of STAT1 (yellow) (right) and IRF3 (red) 24 h after infection of A549 WT cells with RSV (magenta, polyclonal anti-RSV antibody) at an MOI of 0.01. White dotted lines are nuclear outlines determined based on DAPI counterstaining (channel not shown). Bars, 50 μm. (D and E) Activation of STAT1, STAT2, and interferon-stimulated genes (RIG-I, PKR, and OAS1) upon RSV (D) and IAV infection (E) at an MOI of 0.1 at 24 and 48 h postinfection (p.i.) in A549 WT, IFNAR1 KO, IFNLR1 KO, and IFNAR1-IFNLR1 dKO cells. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. RSV F, RSV fusion glycoprotein; IAV NP, IAV nucleoprotein. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of STAT2 by Western Blot

IFN-beta and IFN-lambda mediate STAT1 and STAT2 activation upon RSV and IAV infection in A549 cells. (A and B) ELISA for IFN-beta following RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Open circles mark data points with cytokine concentrations below the detection range. Error bars are standard deviations (SD) from technical replicates. (C) IFN-beta (green) (left) and activation of STAT1 (yellow) (right) and IRF3 (red) 24 h after infection of A549 WT cells with RSV (magenta, polyclonal anti-RSV antibody) at an MOI of 0.01. White dotted lines are nuclear outlines determined based on DAPI counterstaining (channel not shown). Bars, 50 μm. (D and E) Activation of STAT1, STAT2, and interferon-stimulated genes (RIG-I, PKR, and OAS1) upon RSV (D) and IAV infection (E) at an MOI of 0.1 at 24 and 48 h postinfection (p.i.) in A549 WT, IFNAR1 KO, IFNLR1 KO, and IFNAR1-IFNLR1 dKO cells. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. RSV F, RSV fusion glycoprotein; IAV NP, IAV nucleoprotein. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.