Somatostatin R2/SSTR2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Somatostatin R2/SSTR2 in MDA-MB-231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Somatostatin R2/SSTR2 in Human Brain.

Somatostatin R2/ SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using 3 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Somatostatin R2/SSTR2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cingulate cortex)

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: MDA-MB-231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Somatostatin R2/SSTR2

Somatostatin Receptor 2 (SSTR2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTR2 shares 84% aa identity with mouse SSTR2 within the extracellular domains. Isoform B (357 aa) has an alternate C-terminal cytoplasmic region that is 12 aa shorter than that of isoform A (369 aa, reported as 93 kDa). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.

Long Name

Somatostatin Receptor Type 2

Alternate Names

Smstr2, SomatostatinR2, SST2, SSTR2

Entrez Gene IDs

6752 (Human); 20606 (Mouse); 54305 (Rat)

Gene Symbol

SSTR2

UniProt

P30874

Additional Somatostatin R2/SSTR2 Products

Product Documents for Human Somatostatin R2/SSTR2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Somatostatin R2/SSTR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Somatostatin R2/SSTR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4224

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Somatostatin R2/SSTR2 Antibody

Somatostatin R2/SSTR2 in MDA-MB-231 Human Cell Line.