Detects human SMAGP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant rat SMAGP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SMAGP Antibody

Detection of Human SMAGP by Western Blot.

Western blot shows lysates of human lung tissue, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMAGP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

SMAGP in Human Colon.

SMAGP was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SMAGP Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: Human lung tissue, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAGP

SMAGP (small transmembrane and glycosylated protein) is a 25 kDa member of the SMAGP family of proteins. It is expressed on breast, colon and biliary duct epithelium, and likely plays a role in cell-cell adhesion. Human SMAGP is an O-glycosylated, 97 amino acid (aa) type III transmembrane glycoprotein that contains a signal sequenceless N-terminal extracellular region (aa 1-34) plus a 39 aa C-terminal cytoplasmic domain. Over the intra- and extracellular regions combined, human SMAGP is 76% aa identical to mouse SMAGP.

Long Name

Small Transmembrane and Glycosylated Protein

Alternate Names

LOC57228

Entrez Gene IDs

57228 (Human); 207818 (Mouse); 300236 (Rat)

Gene Symbol

SMAGP

UniProt

Q0VAQ4

Additional SMAGP Products

Product Documents for Human SMAGP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SMAGP Antibody

For research use only

Citations
Reviews

Human SMAGP Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3959

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SMAGP Antibodies »

Product Specifications

Immunogen

Specificity