Human SLC1A5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11628
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3085B
Product Specifications
Immunogen
Synthetic Peptide
Accession # Q9JKD8
Accession # Q9JKD8
Specificity
Detects a synthetic peptide specific for human SLC1A5 around amino acid 520 in Direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human SLC1A5 Antibody
Detection of SLC1A5 in Human Prostate.
SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11628) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of SLC1A5 in SW480 Human Cell Line.
SLC1A5 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # MAB11628) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human SLC1A5 Antibody
Application
Recommended Usage
Immunocytochemistry
1-10 µg/mL
Sample: Immersion fixed SW480 human colorectal adenocarcinoma cell line
Sample: Immersion fixed SW480 human colorectal adenocarcinoma cell line
Immunohistochemistry
1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLC1A5
References
- Nachef M, Ali AK, Almutairi SM, Lee SH. Targeting SLC1A5 and SLC3A2/SLC7A5 as a Potential Strategy to Strengthen Anti-Tumor Immunity in the Tumor Microenvironment. Front Immunol. 2021 Apr 19;12:624324. doi: 10.3389/fimmu.2021.624324. PMID: 33953707; PMCID: PMC8089370.
- Chen P, Jiang Y, Liang J, Cai J, Zhuo Y, Fan H, Yuan R, Cheng S, Zhang Y. SLC1A5 is a novel biomarker associated with ferroptosis and the tumor microenvironment: a pancancer analysis. Aging (Albany NY). 2023 Aug 10;15(15):7451-7475. doi: 10.18632/aging.204911. Epub 2023 Aug 10. PMID: 37566748; PMCID: PMC10457057.
Long Name
SLC1A5
Alternate Names
AAAT, ASCT2, ATBO, baboon M7 virus receptor, M7V1, M7VS1, R16, RD114 virus receptor, RDRC
Gene Symbol
Q15758
UniProt
Additional SLC1A5 Products
Product Documents for Human SLC1A5 Antibody
Product Specific Notices for Human SLC1A5 Antibody
For research use only
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