Human Skp2 Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6817AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects human Spk2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Skp2 Alexa Fluor™ Plus 680-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: Skp2
Skp2 (S-phase kinase-associated protein 2; also F-box/LRR protein 1) is a 45-49 kDa member of the F-box protein family. It is known to be part of the SCF (Skp1/Cul1/F-box protein) complex. The SCF complex functions as an E3 ubiquitin-protein ligase that mediates the transfer of ubiquitin from an E2 enzyme to a target substrate. The specificity of the target is determined by Skp2. Molecules targeted for degradation include cell-cycle regulatory proteins that are involved in the G1-S phase transition. Human Skp2 is 424 amino acids (aa) in length. It contains one F-box (aa 94-140) followed by nine consecutive Leu-rich repeats (aa 142-369). There are two potential splice forms that contain substitutions for the same aa sequence. One contains a 4 aa substitution, while a second contains a 56 aa substitution for aa 354-424. Over aa 130-350, human Skp2 shares 84% aa identity with mouse Skp2.
Long Name
S Phase Kinase-associated Protein 2
Alternate Names
FBL1, FBXL1, p45skp2
Gene Symbol
SKP2
UniProt
Additional Skp2 Products
Product Specific Notices for Human Skp2 Alexa Fluor™ Plus 680-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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