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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 5/SIRT5
Met33-Ser310
Accession # Q9NXA8

Specificity

Detects human Sirtuin 5/SIRT5  in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SIRT1, rhSIRT2, and rhSIRT6 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Sirtuin 5/SIRT5

Sirtuin 5 (SIRT5; SIR2-like protein 5) is a 28‑35 kDa class III member of the sirtuin protein family. It is found in the mitochondrial matrix and is involved in ammonia detoxification. In particular, it deacetylates carbamoyl phosphate synthetase 1/CPS1, thus activating it and allowing for ammonia condensation with bicarbonate and ATP. This is a crucial step in urea formation during prolonged fasting or the use of a high-protein diet. Human SIRT5 is 310 amino acids (aa) in length. It possesses a sertuin‑type deacetylase domain (aa 41‑309) that contains an NAD binding motif (Thr276:Glu277:Pro292). There are three potential isoform variants that show aa substitutions at the C‑terminus. One contains a 14 aa substitution for aa 286‑310, a second shows a 15 aa substitution for aa 287‑310, and the third shows a 17 aa substitution for aa 207‑310. Full-length human and mouse SIRT5 share 86% aa identity.

Long Name

Silent Mating Type Information Regulation 5 Homolog

Alternate Names

SIR2L5, SIRT5

Entrez Gene IDs

23408 (Human); 68346 (Mouse); 306840 (Rat)

Gene Symbol

SIRT5

UniProt

Additional Sirtuin 5/SIRT5 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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