Human SIRP alpha/CD172a PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4546P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 602411
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha/CD172a
Gly27-Asn370 (predicted)
Accession # P78324
Gly27-Asn370 (predicted)
Accession # P78324
Specificity
Detects human SIRP alpha/CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta1 and no cross-reactivity with rhSIRP beta2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta1 and no cross-reactivity with rhSIRP beta2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SIRP alpha/CD172a PE-conjugated Antibody
Detection of SIRP alpha/CD172a in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRPa/CD172a PE-conjugated Monoclonal Antibody (Catalog # FAB4546P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human SIRP alpha/CD172a PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.
Long Name
Signal-regulatory Protein alpha
Alternate Names
BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA
Entrez Gene IDs
Gene Symbol
SIRPA
UniProt
Additional SIRP alpha/CD172a Products
Product Documents for Human SIRP alpha/CD172a PE-conjugated Antibody
Product Specific Notices for Human SIRP alpha/CD172a PE-conjugated Antibody
For research use only
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