Skip to main content

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1026113

Product Summary for Human Siglec-15 Alexa Fluor® 647‑conjugated Antibody

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Siglec-15
Phe20-Thr263
Accession # Q6ZMC9

Specificity

Detects human Siglec-15 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Siglec-15 Alexa Fluor® 647‑conjugated Antibody

Detection of Siglec-15 in Tranfectants by Flow Cytometry.

hSiglec-15 HEK/eGFP transfectants were stained with (A) Mouse Anti-Human Siglec-15 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB9227R) or (B) Mouse IgG1 Alexa Fluor® 647-conjugated isotype control Antibody (Catalog # IC002R). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human Siglec-15 Alexa Fluor® 647‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

References

  1. Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
  2. Angata, T. et al. (2007) Glycobiology 17:838.
  3. Takamiya, R. et al. (2013) Glycobiology 23:178.
  4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
  5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
  6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Alternate Names

CD33L3, HsT1361, Siglec15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Gene Symbol

SIGLEC15

UniProt

Additional Siglec-15 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Siglec-15 Alexa Fluor® 647‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...
Loading...