Human Siglec-15 Alexa Fluor® 350-conjugated Antibody

Name: Human Siglec-15 Alexa Fluor® 350-conjugated Antibody
Brand: R&D Systems
SKU: FAB9227U
Price: 367 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB9227U

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Species Reactivity:	Human
Immunogen:	Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 Phe20-Thr263 Accession # Q6ZMC9
Applications:	Flow Cytometry
Label:	Alexa Fluor 350 (Excitation=346 nm, Emission=442 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 1026113
Specificity:	Detects human Siglec-15 in direct ELISAs.

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Applications

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

Item	Value
References	Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914. Angata, T. et al. (2007) Glycobiology 17:838. Takamiya, R. et al. (2013) Glycobiology 23:178. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
Long Name	Sialic Acid Binding Ig-like Lectin 15
Entrez Gene IDs	284266 (Human); 620235 (Mouse); 498888 (Rat)
Alternate Names	CD33 antigen-like 3SIGLEC-15, CD33 molecule-like 3, CD33L3, HsT1361, Siglec-15, Siglec15, sialic acid binding Ig-like lectin 15, sialic acid-binding Ig-like lectin 15

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human Siglec-15 Alexa Fluor® 350-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Background: Siglec-15

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