Detects human Serpin B5/Maspin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Serpin A5, B6, B8 or B9 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human Serpin B5/Maspin Antibody

Detection of Human Serpin B5/Maspin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Serpin B5/Maspin Monoclonal Antibody (Catalog # MAB2218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human Serpin B5/Maspin Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Serpin B5/Maspin knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Serpin B5/Maspin Monoclonal Antibody (Catalog # MAB2218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Serpin B5/Maspin Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin B5/Maspin, see our available Western blot detection antibodies

Knockout Validated

Serpin B5/Maspin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Serpin B5/Maspin knockout HeLa cell line.

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B5/Maspin

Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. The amino acid sequence of human Serpin B5 is 99%, 92%, 89%, 88% and 64% identical to that of chimpanzee, dog, mouse, rat and chicken.

Alternate Names

Maspin, PI5

Entrez Gene IDs

5268 (Human)

Gene Symbol

SERPINB5

UniProt

P36952

Additional Serpin B5/Maspin Products

Product Documents for Human Serpin B5/Maspin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Serpin B5/Maspin Antibody

For research use only

Citations
Reviews

Human Serpin B5/Maspin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2218

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all Serpin B5/Maspin Antibodies »

Product Specifications

Immunogen

Specificity