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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 210326

Product Summary for Human Serpin A5/Protein C Inhibitor Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A5/Protein C
His20-Pro406
Accession # P05154

Specificity

Detects human Serpin A5/Protein C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Serpin A1, A3, F2, C1, or recombinant mouse Serpin A5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Serpin A5/Protein C Inhibitor Antibody

Detection of Serpin A5 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of Serpin A5 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serpin A5 Monoclonal Antibody (Catalog # MAB1266, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human Serpin A5/Protein C Inhibitor Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A5/
Protein C Inhibitor (Catalog # 1266-PI), see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Serpin A5/Protein C Inhibitor (Catalog # 1266-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using MAB1266 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A5/Protein C Inhibitor

Serpin A5 is the a member of the Serpin superfamily and inhibits a variety of serine proteases such as protein C, plasminogen activators, thrombin, factor Xa, several kallekreins and acrosin (1). Serpin A5 is synthesized in the liver and secreted in plasma. It is found in numerous steroid-responsive organs and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. Because of its protease targets and regulated expression patterns, Serpin A5 has been proposed to play a role in processes such as blood coagulation, fertilization and carcinogenesis (2, 3). Similar to Serpins C1 and D1, its thrombin inhibitory activity is enhanced by heparin.

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Palmier, D. et al. (2002) J. Biol. Chem. 277:40950.
  3. Geiger, M. et al. (1996) Immunopharmacology 32:53.

Alternate Names

PAI-3, PCI

Entrez Gene IDs

5104 (Human); 268591 (Mouse)

Gene Symbol

SERPINA5

UniProt

Additional Serpin A5/Protein C Inhibitor Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Serpin A5/Protein C Inhibitor Antibody

For research use only

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