Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF12681) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A1/a1-Antitrypsin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Serpin A1/ alpha1-Antitrypsin Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Human Plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A1/alpha 1-Antitrypsin

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe serpin A1 deficiency (3).

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Barbour, K.W. et al. (2002) Genomics 80:515.
Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Entrez Gene IDs

5265 (Human)

Gene Symbol

SERPINA1

UniProt

P01009

Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Human Serpin A1/ alpha1-Antitrypsin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Serpin A1/ alpha1-Antitrypsin Antibody

For research use only

Citations
Reviews

Human Serpin A1/ alpha1-Antitrypsin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF12681

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Serpin A1/alpha 1-Antitrypsin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Serpin A1/ alpha1-Antitrypsin Antibody

Detection of Human Serpin A1/ alpha1‑Antitrypsin by Western Blot.