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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A
Gly32-His683
Accession # Q9H3S1

Specificity

Detects human Semaphorin 4A in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Semaphorin 4A is observed, and less than 1% cross-reactivity with recombinant human (rh) Semaphorin 4B, rhSemaphori

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Semaphorin 4A

Sema4A (Semaphorin 4A; also Sema B) is a class IV member of the semaphorin family of proteins. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. It reportedly binds to Tim-2 and Plexin D1 and B1. In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype. In the nervous system, it induces growth cone collapse in neurons. Mature human Sema4A is an 80 kDa (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa 33-761). It contains a 651 aa extracellular region (aa 33-683) that is characterized by the presence of one Sema domain (aa 36-494), a PSI region (aa 496-528), and an Ig-like C2-type domain (aa 573-631). The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747. There are two potential splice variants. One contains a 37 aa substitution for aa 293-328, while a second contains an alternative start site at Met100 coupled to a deletion of aa 122-154. Sema4A is reportedly cleaved to generate both a monomeric and a  dimeric soluble isoforms. Over aa 32-683, human Sema4A shares 86% aa identity with mouse Sema4A.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Gene Symbol

SEMA4A

UniProt

Additional Semaphorin 4A Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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