Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and EL-4 mouse lymphoblast cell line. Gels were loaded with 20 µg of HepG2 and 30 µg of EL-4 cytoplasmic (Cyto) extracts and 10 µg of HepG2 and 15 µg of EL-4 nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SART1 Monoclonal Antibody (Catalog # MAB8034) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SART1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SART1 in HepG2 Human Cell Line.

SART1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human SART1 Monoclonal Antibody (Catalog # MAB8034) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SART1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and EL‑4 mouse lymphoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SART1

Hypoxia-associated factor (HAF), also known as SART1, is a widely expressed 125 kDa intracellular protein that promotes assembly of the U2-type pre-mRNA spliceosome. SART1 is also an E3 ubiquitin ligase that promotes the ubiquitination and degradation of HIF-1 alpha. In contrast, it enhances the transcriptional activity of HIF-2 alpha. SART1 is upregulated in many tumor types and enables tumor growth during chronic hypoxia. Within amino acids 647-800, human SART1 shares 100% aa sequence identity with mouse and rat SART1.

Long Name

Squamous cell carcinoma antigen recognized by T cells

Alternate Names

Ara1, HAF, HOMS1, IgE Autoantigen, SART1259, SNRNP110, Snu66

Entrez Gene IDs

9092 (Human); 20227 (Mouse); 29678 (Rat)

Gene Symbol

SART1

UniProt

O43290

Additional SART1 Products

Product Documents for Human SART1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SART1 Antibody

For research use only

Citations
Reviews

Human SART1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8034

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SART1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SART1 Antibody

Detection of Human and Mouse SART1 by Western Blot.