Human RFC1 Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6457AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects human RFC1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human RFC1 Alexa Fluor™ Plus 680-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: RFC1
RFC1 (Replication factor C subunit 1; also RFC140) is a 140‑150 kDa, ubiquitously expressed member of the activator 1 large subunit family of proteins. It is one of five RFC complex subunits that participate in the early stages of DNA replication. Following the initiation of DNA synthesis by pol alpha/primase, an RFC complex displaces pol alpha at the replication fork, and recruits a PCNA homotrimeric complex to this site. This interacts with pol delta to extend the newly‑formed DNA strand. Human RFC1 is 1148 amino acids (aa) in length. It contains a phosphorylated-DNA binding region (aa 366‑477), a PCNA binding domain (aa 478‑712), an ATPase site (aa 643‑772), and an NLS (aa 1120‑1124). Caspase-3 cleaves RFC-1 into 97, 73 and 65 kDa fragments during apoptosis. There is one potential alternative start site at Met117. Over aa 800‑1093, human RFC1 shares 90% aa identity with mouse RFC1.
Long Name
Replication Factor C 1
Alternate Names
MHCBFB, PO-GA, RECC1, RFC140
Gene Symbol
RFC1
UniProt
Additional RFC1 Products
Product Specific Notices for Human RFC1 Alexa Fluor™ Plus 680-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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