Human RENT1/UPF1 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5879S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RENT1/UPF1
Asp351-Arg600
Accession # NP_002902
Asp351-Arg600
Accession # NP_002902
Specificity
Detects human RENT1/UPF1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human RENT1/UPF1 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: RENT1/UPF1
UPF1 (Up-frameshift mutation-1; also RENT1, SMG-2 and NORF1) is a 125-130 kDa member of the DNA2/NAM7 helicase family of molecules. It is ubiquitously expressed, and promotes the turnover of mRNA containing premature stop codons. UPF1 is a cytoplasmic factor that contributes to the formation of a UPF complex. During the translation of properly transcribed mRNA, unphosphorylated UPF1 is blocked from interacting with eRF3, a translation initiation factor. In the presence of an improper stop codon, a detection complex termed EJC initiates phosphorylation of UPF1, allowing it to bind to eRF3 and repress translation. Human UPF1 is 1129 amino acids (aa) in length. It contains one C2H2-type Zn finger motif (aa 131-159), an NTPase domain (aa 486-545) and three phosphorylation sites at Ser1107/1127/1129. There are two potential isoforms that show deletions of aa 54-58 and 353-363, respectively. Over aa 351-600, human UPF1 shares 99% aa identity with mouse UPF1.
Long Name
Regulator of Nonsense Transcripts 1
Alternate Names
NORF1, UPF1
Gene Symbol
UPF1
UniProt
Additional RENT1/UPF1 Products
Product Specific Notices for Human RENT1/UPF1 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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