Detects human Reg1A in Western blots. Shows 5% cross-reactivity with recombinant human (rh) Reg1B and no cross-reactivity with rhReg3A, recombinant mouse Reg1, or recombinant rat Reg1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human Reg1A Antibody

Detection of Reg1A in AGS Human Cell Line by Flow Cytometry.

AGS human gastric adenocarcinoma cell line was stained with Rat Anti-Human Reg1A Monoclonal Antibody (Catalog # MAB4937, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human Reg1A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: AGS human gastric adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human Reg1A

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg1A

Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16‑18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet beta-cells and intestinal villi. It is up‑regulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65%‑68% aa sequence identity with mouse and rat Reg1A.

Long Name

Regenerating Islet-derived 1A

Alternate Names

ICRF, PSP, PTP

Entrez Gene IDs

5967 (Human)

Gene Symbol

REG1A

UniProt

P05451

Additional Reg1A Products

Product Documents for Human Reg1A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Reg1A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Reg1A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4937

Key Product Details

Species Reactivity

Validated:

Cited:

Applications