Detects rat TrkA when phosphorylated at Y785 in Western blots. Reactivity with human Phospho-TrkA is expected due to 100% homology with the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Rat Phospho-TrkA (Y785) Antibody

Detection of Rat Phospho-TrkA (Y785) by Western Blot.

Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with the indicated concentrations of Recombinant Rat beta-NGF (Catalog # 556-NG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human/Rat Phospho-TrkA (Y785) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5479), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-TrkA (Y785) at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Rat Phospho-TrkA (Y785) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: PC-12 rat adrenal pheochromocytoma cell line treated with Recombinant Rat beta-NGF (Catalog # 556-NG)

Reviewed Applications

Read 2 reviews rated 5 using AF5479 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

The receptor tyrosine kinase TrkA binds nerve growth factor (NGF) with low affinity, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires a complex formed between TrkA and the p75 NGF receptor. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues, including Y490, Y751 and Y785. Phosphorylation of TrkA at Y785 creates a binding site for phospholipase C-gamma (PLC-gamma), which catalyzes the cleavage of substrate PIP2 to generate the second messengers DAG and IP3.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat); 102129364 (Cynomolgus Monkey)

Gene Symbol

NTRK1

Additional TrkA Products

Product Documents for Human/Rat Phospho-TrkA (Y785) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat Phospho-TrkA (Y785) Antibody

For research use only

Citations
Reviews

Human/Rat Phospho-TrkA (Y785) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5479

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications