Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and NRK rat normal kidney cells. PVDF membrane was probed with 1 µg/mL of Human/Rat MAD2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MAD2L1 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

MAD2L1 in A549 Human Cell Line.

MAD2L1 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Rat MAD2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4005) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat MAD2L1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and NRK rat normal kidney cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAD2L1

MAD2L1 (Mitotic arrest deficient protein 2) is a component of the spindle-attachment checkpoint mechanism that monitors kinetochore spindle attachment and leads to the subsequent arrest in early metaphase by its recruitment to unattached kinetochores. The transition from metaphase to anaphase requires the association of the anaphase promoting complex/cyclosome (APC/C) with Cdc20 leading to the ubiquitylation and subsequent degradation of Pds1/Securin. This transition is delayed by the inhibitory association of MAD2L1 with Cdc20. MAD2L1 has also been shown to be a direct E2F target and as such is aberrantly expressed in cells with retinoblastoma pathway defects.

Long Name

MAD2 Mitotic Arrest Deficient-Like 1

Alternate Names

HSMAD2

Entrez Gene IDs

4085 (Human); 56150 (Mouse)

Gene Symbol

MAD2L1

UniProt

Q13257

Additional MAD2L1 Products

Product Documents for Human/Rat MAD2L1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat MAD2L1 Antibody

For research use only

Citations
Reviews

Human/Rat MAD2L1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4005

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MAD2L1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Rat MAD2L1 Antibody

Detection of Human/Rat MAD2L1 by Western Blot.