Detects human and rat IkB-beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IkB-alpha or rhIkB-epsilon is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Rat IkB-beta Antibody

Detection of Human IkB‑ beta by Western Blot.

Western blot shows recombinant human Ik beta-a, IkB-beta, and IkB-e (20 ng/lane). PVDF mem-brane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Mono-clonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Human/Rat IkB-beta was detected at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of Human/Rat IkB‑ beta by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Monoclonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific band for Human/Rat IkB-beta were detected at approximately 48 kDa and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Rat IkB-beta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-beta

NF kappaB inhibitor beta (IkB-beta) inhibits the NF kappaB complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-beta is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-beta degradation, the NF kappaB complex is able to translocate to the nucleus and activate transcription.

Long Name

I-kappa-B-beta

Alternate Names

IkBbeta, NFKBIB, TR-interacting protein 9

Entrez Gene IDs

4793 (Human); 18036 (Mouse); 81525 (Rat)

Gene Symbol

NFKBIB

UniProt

Q15653

Additional IkB-beta Products

Product Documents for Human/Rat IkB-beta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat IkB-beta Antibody

For research use only

Citations
Reviews

Human/Rat IkB-beta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3425

Key Product Details

Species Reactivity

Validated:

Cited:

Applications