Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, Hep3B human hepatocellular carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Rat FABP1/L‑FABP by Simple Western^TM.

Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29643) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Rat FABP1/L-FABP Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody for human FABP1/L-FABP when paired with Mouse Anti-Human FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29642).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Simple Western

10 µg/mL
Sample: Rat Liver Tissue

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

P07148

Additional FABP1/L-FABP Products

Product Documents for Human/Rat FABP1/L-FABP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat FABP1/L-FABP Antibody

For research use only

Citations
Reviews

Human/Rat FABP1/L-FABP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB29643

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FABP1/L-FABP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Rat FABP1/L-FABP Antibody

Detection of Human and Rat FABP1/L‑FABP by Western Blot.

Detection of Rat FABP1/L‑FABP by Simple WesternTM.