Human Pyruvate Dehydrogenase Phosphatase/PDP1 Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7260T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pyruvate Dehydrogenase Phosphatase/PDP1
Ala72-Glu537
Accession # Q9P0J1
Ala72-Glu537
Accession # Q9P0J1
Specificity
Detects human Pyruvate Dehydrogenase Phosphatase/PDP1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Pyruvate Dehydrogenase Phosphatase/PDP1
PPM2C (Protein phosphatase mitochondrial 2C; also PDP1) is a 52-54 kDa member of the PP2C family of enzymes. It is expressed in skeletal muscle and adipocytes, and represents one of two subunits that comprise PDP (pyruvate dehydrogenase phosphatase). PPMC2 is found within the mitochondrial matrix space where, as part of PDP, it dephosphorylates the E1 component of the pyruvate dehydrogenase complex, resulting in its activation, and the downstream generation of acetyl-CoA and NADH from pyruvate. Human PPM2C is 537 amino acids (aa) in length. It contains a mitochondrion targeting sequence (aa 1-71), followed by a mature region that possesses a catalytic domain between aa 109-440. There are three potential isoform variants. Two contain alternative start sites 25 and 59 aa upstream of the standard start site, respectively. A third shows a 12 aa substitution for aa 105-116. Over aa 72-537, human PPM2C shares 99% aa identity with mouse PPM2C.
Alternate Names
PDH, PDP1, PDPC 1, PPM2C
Gene Symbol
PDP1
UniProt
Additional Pyruvate Dehydrogenase Phosphatase/PDP1 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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