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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 677939

Product Specifications

Immunogen

E. coli-derived recombinant human PTHLH/PTHrP
Ala37-Arg175
Accession # P12272

Specificity

Detects human PTHLH/PTHrP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human PTHLH/PTHrP Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PTHLH/PTHrP

The parathyroid hormone-like hormone (PTHLH), also known as PTHrP, has been recognized in patients suffering from tumors associated with the common neoplastic syndrome humoral hypercalcemia of malignancy (HHM). The 18 kDa protein is a 177 amino acid (aa) precursor that belongs to the parathyroid hormone family. It contains a 24 aa signal sequence followed by a 10 aa propeptide (aa 25-34). The mature chain runs from aa 37-177. Of note, residues 108-129 constitute a nuclear localization signal (NLS) providing evidence that the protein not only acts as a secreted factor, but in an intracrine manner as well. A second isoform is missing aa 176-177, and a 34 aa substitution for aa 176-177 produces a third isoform. There is a strong homology displayed between PTHLH and human parathyroid hormone (PTH) among the first 13 aa. Studies show that the similarity in this area is 67% at the aa and nucleotide levels. Indeed, eight of the first 13 aa of PTHLH and PTH are identical. Human PTHLH and mouse PTHLH share 86% aa sequence identity.

Long Name

Parathyroid Hormone-like Hormone

Alternate Names

BDE2, HHM, Osteostatin, PTHRP

Entrez Gene IDs

5744 (Human); 19227 (Mouse); 24695 (Rat)

Gene Symbol

PTHLH

UniProt

Additional PTHLH/PTHrP Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PTHLH/PTHrP Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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