Human/Primate MMP-9 Biotinylated Antibody

Name: Human/Primate MMP-9 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF911

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF911

Citations (6)

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Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human, Primate
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Applications:	ELISA Detection, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human and primate MMP-9 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rmMMP-9, rhMMP-1, -2, -3, -7, -8, -10, -12, -13, and -14 is observed.

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Applications for Human/Primate MMP-9 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human MMP‑9 (Catalog # 911-MP)

Human/Primate MMP-9 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human/Primate MMP-9 Antibody (Catalog # MAB936)

Capture Reagent: Human/Primate MMP‑9 Antibody (Catalog # MAB936R)

Standard: Recombinant Human MMP-9 Protein, CF (Catalog # 911-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Item	Value
Long Name	Matrix Metalloproteinase 9
Entrez Gene IDs	4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names	92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, GELB, Gelatinase B, MANDP2, MMP-9, MMP9, macrophage gelatinase, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, type V collagenase

Citations for Human/Primate MMP-9 Biotinylated Antibody (6)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Primate MMP-9 Biotinylated Antibody include:

Species: Human
Sample Types: Plasma
Applications: ELISA Detection

L Tiainen et al. (2019), Low Plasma IL-8 Levels During Chemotherapy Are Predictive of Excellent Long-Term Survival in Metastatic Breast Cancer Clin. Breast Cancer, 0(0).
PMID: 31029558
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

Soumyabrata Roy (2016), Activation of D2 dopamine receptors in CD133+ve cancer stem cells in non-small cell lung carcinoma inhibits proliferation, clonogenic ability and invasiveness of these cells J. Biol. Chem, 0(0).
PMID: 27920206
Species: Human
Sample Types: Beads
Applications: Luminex Development

Walter Maetzler et al. (2016), GDF15/MIC1 and MMP9 Cerebrospinal Fluid Levels in Parkinson's Disease and Lewy Body Dementia. PLoS ONE, 0(0):1932-6203.
PMID: 26938614
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

Ohlsson S et al. (2014), Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies. Clin Exp Immunol, 176(3):363-72.
PMID: 24666336
Species: Human
Sample Types: Serum
Applications: Luminex Development

Madsen EL et al. (2009), Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects. Metab. Clin. Exp., 58(7):946-53.
PMID: 19409578
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development

Gonzalez RM et al. (2008), Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 7(6):2406-14.
PMID: 18422355

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Showing 1 - 6 of 6 citations Showing All

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