Human/Primate BMP-9 Biotinylated Antibody

Name: Human/Primate BMP-9 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3209

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF3209

Citations (5)

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Key Product Details

Species Reactivity:	Human, Primate
Immunogen:	E. coli-derived recombinant human BMP-9 Ser320-Arg429 Accession # Q9UK05
Applications:	ELISA Detection, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human and primate BMP-9 in Western blots and ELISAs. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) BMP‑3, rhBMP-4, rhBMP-5, rhBMP-6, rhBMP-7, and rhBMP-10 is observed.

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Applications for Human/Primate BMP-9 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human BMP‑9 (Catalog # 3209-BP)

Human/Primate BMP-9 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human/Mouse/Primate BMP‑9 Antibody (Catalog # MAB3209)

Standard: Recombinant Human BMP-9 Protein (Catalog # 3209-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

Item	Value
References	Chen, D. et al. (2004) Growth Factors 22:233. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111. Song, J.J. et al. (1995) Endocrinology 136:4293. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937. Chen, C. et al. (2003) Nat. Biotechnol. 21:294. Lopez-Coviella, I. et al. (2000) Science 289:313. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
Long Name	Bone Morphogenetic Protein 9
Entrez Gene IDs	2658 (Human); 12165 (Mouse)
Alternate Names	BMP-9, BMP9, BMP9BMP-9Bone morphogenetic protein 9, GDF-2, GDF2, growth differentiation factor 2, growth/differentiation factor 2

Citations for Human/Primate BMP-9 Biotinylated Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Primate BMP-9 Biotinylated Antibody include:

Species: Human
Sample Types: Plasma
Applications: ELISA (detection)

NE Owen et al. (2020), Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis EBioMedicine, 56(0):102794.
PMID: 32454407
Species: Mouse
Sample Types: Plasma
Applications: ELISA Detection

A Desroches- et al. (2019), Differential Consequences of Bmp9 Deletion on Sinusoidal Endothelial Cell Differentiation and Liver Fibrosis in 129/Ola and C57BL/6 Mice Cells, 8(9).
PMID: 31540222
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

S Gräf et al. (2018), Identification of rare sequence variation underlying heritable pulmonary arterial hypertension Nat Commun, 9(1):1416.
PMID: 29650961
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development (Detection)

K Breitkopf- et al. (2017), BMP-9 interferes with liver regeneration and promotes liver fibrosis Gut, 0(0).
PMID: 28336518
Species: Human
Sample Types: Protein
Applications: ELISA Development (Detection)

Y Kienast et al. (2016), Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains J. Biol. Chem, 291(7):3395-410.
PMID: 26677222

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Showing 1 - 5 of 5 citations Showing All

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