What is the half-life of a recombinant or natural protein in culture at 37 °C?

Name: Recombinant Human BMP-9 Protein
Brand: R&D Systems
SKU: 3209-BP
Rating: 4 (2 reviews)

R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human BMP-9 Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3209-BP

Citations (47)

(2)

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Key Product Details

Source:	CHO
Accession #:	Q9UK05
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human BMP-9 Protein

Product Specifications for Recombinant Human BMP-9 Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein Ser320-Arg429
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser320
Predicted Molecular Mass	12.1 kDa (monomer)
SDS-PAGE	13 kDa, reducing conditions
Activity	Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.4-1.6 ng/mL.

Scientific Data Examples for Recombinant Human BMP-9 Protein

	Recombinant Human BMP-9 Protein Bioactivity Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is 0.4-1.6 ng/mL.
	Recombinant Human BMP-9 Protein SDS-PAGE 1 μg/lane of Recombinant Human BMP-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 23 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3209-BP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

3209-BP/CF

Carrier Free

Formulation	Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	It is recommended that sterile 4 mM HCl be added to the vial to prepare a working stock solution of no less than 100 μg/mL. The carrier-free protein should be used immediately upon dilution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4 - 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8 - 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

Item	Value
References	Chen, D. et al. (2004) Growth Factors 22:233. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111. Song, J.J. et al. (1995) Endocrinology 136:4293. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937. Chen, C. et al. (2003) Nat. Biotechnol. 21:294. Lopez-Coviella, I. et al. (2000) Science 289:313. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
Long Name	Bone Morphogenetic Protein 9
Entrez Gene IDs	2658 (Human); 12165 (Mouse)
Alternate Names	BMP-9, BMP9, BMP9BMP-9Bone morphogenetic protein 9, GDF-2, GDF2, growth differentiation factor 2, growth/differentiation factor 2

Citations for Recombinant Human BMP-9 Protein (47)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BMP-9 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

C Ivaldo et al. (2023), Oxidative stress-induced MMP- and gamma-secretase-dependent VE-cadherin processing is modulated by the proteasome and BMP9/10 Scientific Reports, 13(1):597.
PMID: 36631513
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

F Kawecki et al. (2022), Preclinical Evaluation of BMP-9-Treated Human Bone-like Substitutes for Alveolar Ridge Preservation following Tooth Extraction International Journal of Molecular Sciences, 23(6).
PMID: 35328724
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Addante et al. (2022), BMP9 Promotes an Epithelial Phenotype and a Hepatocyte-like Gene Expression Profile in Adult Hepatic Progenitor Cells Cells, 11(3).
PMID: 35159174
Species: Human
Sample Types: Organoid
Applications: Bioassay

DL Marvin et al. (2022), Dynamic Visualization of TGF-beta/SMAD3 Transcriptional Responses in Single Living Cells Cancers, 14(10).
PMID: 35626109
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Shonibare et al. (2022), Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer Oncogene, 40(4):111066.
PMID: 35905726
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

M Fujioka-Ko et al. (2021), Osteoinductive potential of recombinant BMP-9 in bone defects of mice treated with antiresorptive agents International journal of oral and maxillofacial surgery, 0(0).
PMID: 34454793
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Q Jiang et al. (2021), BMP9 promotes methionine- and choline-deficient diet-induced nonalcoholic steatohepatitis in non-obese mice by enhancing NF-&kapppaB dependent macrophage polarization International immunopharmacology, 96(0):107591.
PMID: 33812253
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

X Wang et al. (2020), CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway J Cancer, 11(2):272-283.
PMID: 31897223
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Alinejad et al. (2020), Both Human Hematoma Punctured from Pelvic Fractures and Serum Increase Muscle Resident Stem Cells Response to BMP9: A Multivariate Statistical Approach J Clin Med, 9(4).
PMID: 32325892
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

A Addante et al. (2020), A Signaling Crosstalk between BMP9 and HGF/c-Met Regulates Mouse Adult Liver Progenitor Cell Survival Cells, 9(3).
PMID: 32204446
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Tao et al. (2020), BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells J Biol Chem, 0(0).
PMID: 33097593
Species: Mouse
Sample Types: Beads
Applications: In Vivo

L Yu et al. (2019), BMP9 stimulates joint regeneration at digit amputation wounds in mice Nat Commun, 10(1):424.
PMID: 30723209
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

G Drouin et al. (2019), Muscle injury-induced hypoxia alters the proliferation and differentiation potentials of muscle resident stromal cells Skelet Muscle, 9(1):18.
PMID: 31217019
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Cao et al. (2019), Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives Stem Cell Reports, 12(6):1282-1297.
PMID: 31189095
Applications: Western Blot

E Tillet et al. (2018), A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma J. Biol. Chem., 0(0).
PMID: 29789425
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Schlereth et al. (2018), The transcriptomic and epigenetic map of vascular quiescence in the continuous lung endothelium Elife, 7(0).
PMID: 29749927
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Subileau et al. (2018), Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation Stem Cell Reports, 0(0).
PMID: 30595547
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Tian et al. (2018), Endoglin interacts with VEGFR2 to promote angiogenesis FASEB J., 0(0):fj201700867RR.
PMID: 29401587
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Akatsu et al. (2017), Dual targeting of vascular endothelial growth factor and bone morphogenetic protein-9/10 impairs tumor growth through inhibition of angiogenesis Cancer Sci, 108(1):151-155.
PMID: 28133920
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

W Abdullahi et al. (2017), Bone morphogenetic protein-9 increases the functional expression of organic anion transporting polypeptide 1a4 at the blood-brain barrier via the activin receptor-like kinase-1 receptor J. Cereb. Blood Flow Metab., 0(0):271678X177029.
PMID: 28387157
Applications: ELISA (Standard)

K Breitkopf- et al. (2017), BMP-9 interferes with liver regeneration and promotes liver fibrosis Gut, 0(0).
PMID: 28336518
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CG Mitrofan et al. (2017), Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2 J. Biol. Chem., 0(0).
PMID: 28646109
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Sakamoto et al. (2017), Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology Biochem Biophys Rep, 11(0):33-39.
PMID: 28955765
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

SR Pasricha et al. (2017), Hepcidin is regulated by promoter-associated histone acetylation and HDAC3 Nat Commun, 8(1):403.
PMID: 28864822
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MA Lauzon et al. (2017), Peptides derived from the knuckle epitope of BMP-9 induce the cholinergic differentiation and inactivate GSk3beta in human SH-SY5Y neuroblastoma cells Sci Rep, 7(1):4695.
PMID: 28680159
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Tachida et al. (2017), Mutual interaction between endothelial cells and mural cells enhances BMP9 signaling in endothelial cells Biol Open, 6(3):370-380.
PMID: 28298363
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), MxA Is a Novel Regulator of Endosome-Associated Transcriptional Signaling by Bone Morphogenetic Proteins 4 and 9 (BMP4 and BMP9) PLoS ONE, 11(11):e0166382.
PMID: 27875556
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Kraehling J et al. (2016), Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells. Nat Commun, 7(0):13516.
PMID: 27869117
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential J Clin Med, 5(12).
PMID: 27916889
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Bone Morphogenetic Protein 9 Enhances Lipopolysaccharide-Induced Leukocyte Recruitment to the Vascular Endothelium J Immunol, 0(0).
PMID: 27647829
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Levet S et al. (2015), BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus. Proc Natl Acad Sci U S A, 112(25):E3207-15.
PMID: 26056270
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Young K et al. (2015), BMP9 Crosstalk with the Hippo Pathway Regulates Endothelial Cell Matricellular and Chemokine Responses. PLoS ONE, 10(4):e0122892.
PMID: 25909848
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tate C et al. (2015), A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology. PLoS ONE, 10(4):e0125697.
PMID: 25919028
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Woltje K et al. (2015), Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells. PLoS ONE, 10(3):e0120547.
PMID: 25799559
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Pan C et al. (2014), Src-mediated post-translational regulation of endoglin stability and function is critical for angiogenesis. J Biol Chem, 289(37):25486-96.
PMID: 25070888
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Olsen O et al. (2014), Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin. Blood Cancer J, 4(0):e196.
PMID: 24658374
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu Y et al. (2013), Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits. Nat Biotechnol, 31(5):440-7.
PMID: 23604284
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2012), BMP9 and BMP10 are critical for postnatal retinal vascular remodeling. Blood, 119(25):6162-71.
PMID: 22566602
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Wu et al. (2012), Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs). PLoS ONE, 7(9):e46307.
PMID: 23029472
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Young et al. (2012), BMP9 regulates endoglin-dependent chemokine responses in endothelial cells. Blood, 120(20):4263-73.
PMID: 23018639
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay

Yao Y et al. (2012), Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium. Blood, 119(21):5037-47.
PMID: 22474252
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Castonguay R et al. (2011), Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 286(34):30034-46.
PMID: 21737454
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ricard N et al. (2010), Functional analysis of the BMP9 response of ALK1 mutants from HHT2 patients: a diagnostic tool for novel ACVRL1 mutations. Blood, 116(9):1604-12.
PMID: 20501893
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Herrera B et al. (2009), A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol., 10(0):20.
PMID: 19298647
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

David L et al. (2007), Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells. Blood, 109(5):1953-61.
PMID: 17068149
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lin L et al. (2007), Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through hemojuvelin and BMP2/4. Blood, 110(6):2182-9.
PMID: 17540841

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Recombinant Human BMP-9 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 11/20/2019

Stimulated cell line with BMP-9 protein to induce pSmad 1/5/8 signaling
Recombinant Human BMP-9 Protein

Name: Justin Provencher

Application: CellProlif

Reason for Rating: This product exceeded expectations and produced beautiful data from my experiment. I have already purchased more vials.

Verified Customer | Posted 10/09/2019

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FAQs for Recombinant Human BMP-9 Protein

What is the half-life of a recombinant or natural protein in culture at 37 °C?

R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human BMP-9 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human BMP-9 Protein

Scientific Data Examples for Recombinant Human BMP-9 Protein

Formulation, Preparation and Storage

Carrier Free

3209-BP

Carrier

3209-BP/CF

Carrier Free

Reconstitution Calculator

Background: BMP-9

Citations for Recombinant Human BMP-9 Protein (47)

Customer Reviews for Recombinant Human BMP-9 Protein (2)

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Recombinant Human BMP-9 Protein

Recombinant Human BMP-9 Protein

FAQs for Recombinant Human BMP-9 Protein

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