Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3485

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BAF3485
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Angiopoietin-like Protein 4/ANGPTL4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 4
Leu165-Ser406
Accession # Q9BY76

Specificity

Detects human ANGPTL4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ANGPTL3, rhAngiopoietin-1, -2, -3, and -4 is observed.In Western blots, approximately 1% cross-reactivity with rhANGPLT4 N-terminal Fragment is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images

Human Angiopoietin-like Protein 4 / ANGPTL4 ELISA Standard Curve

Human Angiopoietin-like Protein 4 / ANGPTL4 ELISA Standard Curve

Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN) was serially diluted and captured by Goat Anti-Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of Human Angiopoietin-like Protein 4/ANGPTL4 by ELISA

Detection of Human Angiopoietin-like Protein 4/ANGPTL4 by ELISA

Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN) was serially diluted and captured by Goat Anti-Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Human Angiopoietin-like 4 DuoSet ELISA

Human Angiopoietin-like 4 DuoSet ELISA

Recombinant Human Angiopoietin-like Protein 4/ANGPTL4 (Catalog # 4487-AN) was serially diluted and captured by Goat Anti-Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3485) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Angiopoietin-like 4/ANGPTL4 (Catalog # 4487-AN)

Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate Angiopoietin-like Protein 4/ANGPTL4 Antibody (Catalog # AF3485)
  • Standard: Recombinant Human Angiopoietin-like 4 Protein, CF (Catalog # 4487-AN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 4/ANGPTL4

ANGPTL4, also known as PPAR gamma angiopoietin-related protein (PGAR), hepatic fibrinogen/angiopoietin-related protein (HFARP) and fasting-induced adipose factor (FIAF), is a secreted protein that shares structural homology with angiopoietins. It contains an N-terminal coiled-coil region that mediates covalent homo-oligomerization and a C-terminal fibrinogen-like domain. ANGPTL4 undergoes proteolytic processing and releases the C-terminal domain, which circulates as a monomer. At least 2 additional splice isoforms exist. ANGPTL4 is most highly expressed in adipose tissues. Its expression is up-regulated in endothelial cells and cardiomyocytes during hypoxia. ANGPTL4 is involved in the regulation of lipid and glucose metabolism. It has also been associated with angiogenesis. The amino acid sequence of human ANGPTL4 is 81%, 76% and 81% identical to that of porcine, mouse and canine ANGPTL4, respectively.

Alternate Names

Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Gene Symbol

ANGPTL4

UniProt

Q9BY76

Additional Angiopoietin-like Protein 4/ANGPTL4 Products

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Certificate of Analysis

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Product Specific Notices

For research use only

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