Detects human PRDM14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse PRDM14, recombinant human (rh) ZNF24, rhZNF143, rhZNF206 or rhZNF281 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human PRDM14 Antibody

Detection of Human PRDM14 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PRDM14 Monoclonal Antibody (Catalog # MAB6175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRDM14 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PRDM14 in BG01V Human Cell Line.

PRDM14 was detected in fixed BG01V human embryonic stem cells grown on irradiated MEF cells using Human PRDM14 Monoclonal Antibody (Catalog # MAB6175) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PRDM14 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells grown on iMEFs

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRDM14

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400-568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.

Long Name

PR Domain Containing 14

Alternate Names

MGC59730, PFM11, PR domain containing 14, PR domain zinc finger protein 14, PR domain-containing protein 14, PR-domain zinc finger protein 14

Entrez Gene IDs

63978 (Human); 383491 (Mouse)

Gene Symbol

PRDM14

UniProt

Q9GZV8 (Human)

Additional PRDM14 Products

Product Documents for Human PRDM14 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PRDM14 Antibody

For research use only

Citations
Reviews

Human PRDM14 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6175

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PRDM14 Antibodies »

Product Specifications

Immunogen

Specificity