Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PP4 Catalytic Subunit Monoclonal Antibody (Catalog # MAB5074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP4 Catalytic Subunit at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PP4 Catalytic Subunit Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP4

The catalytic subunit of Protein Phosphatase 4 (PP4), also called PPX, is a serine/threonine protein phosphatase with about 65% homology to PP2A. Although PP4 has the same heterotrimeric subunit structure as PP2A and can use many of the PP2A regulatory subunits, PP4 also has its own unique scaffolding and regulatory subunits. PP4 is largely found in the centrosome of the nucleus where it may play a role in modulating transcription factor phosphorylation by JNK. In Drosophila, cmm mutants have low levels of PP4 and are unable to complete mitosis due to a deficit in microtubule assembly.

Long Name

Protein Phosphatase 4

Alternate Names

PP4C, PPH3, PPP4C, PPX

Entrez Gene IDs

5531 (Human); 56420 (Mouse); 171366 (Rat)

Gene Symbol

PPP4C

UniProt

P60510

Additional PP4 Products

Product Documents for Human PP4 Catalytic Subunit Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PP4 Catalytic Subunit Antibody

For research use only

Citations
Reviews

Human PP4 Catalytic Subunit Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5074

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all PP4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PP4 Catalytic Subunit Antibody

Detection of Human PP4 Catalytic Subunit by Western Blot.