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Human PON1 Antibody
Catalog # AF5816 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Summary for Human PON1 Antibody
Immunogen
E. coli-derived recombinant human PON1
Ala30-Leu355
Accession # P27169
Ala30-Leu355
Accession # P27169
Specificity
Detects human PON1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PON1 Antibody
Detection of Human PON1 by Western Blot.
Western blot shows lysates of human liver tissue . PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human PON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5816) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, an enriched HDL/LDL fraction obtained from ultracentrifugation of human plasma was included. A specific band for PON1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human PON1 by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for PON1 at approximately 52 kDa (as indicated) using 5 µg/mL of Goat Anti-Human PON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5816) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human PON1 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Western Blot
0.5 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF5816 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PON1
Product Documents for Human PON1 Antibody
Product Specific Notices for Human PON1 Antibody
For research use only
Customer Reviews for Human PON1 Antibody (1)
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