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Human Plexin D1 Antibody
Catalog # MAB41601 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 752815
Product Summary for Human Plexin D1 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Plexin D1
Leu47-Ala1271
Accession # Q9Y4D7
Leu47-Ala1271
Accession # Q9Y4D7
Specificity
Detects human Plexin D1 in ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Plexin D1 Antibody
Detection of Human Plexin D1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Plexin D1 Monoclonal Antibody (Catalog # MAB41601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Plexin D1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and IMR‑32 human neuroblastoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and IMR‑32 human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3 using MAB41601 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin D1
Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors (1, 2). Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins (1). The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and ~84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron (1, 2). Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus (3). Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct (4). Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities (4, 5). Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development (2, 6). In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal (7, 8). Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E (9). Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands (10, 11).
References
- Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
- Van Der Zwaag, B. et al. (2002) Dev. Dyn. 225:336.
- Entrez protein Accession # Q9Y4D7, EAW79239, EAW79240.
- Gu, C. et al. (2005) Science 307:265.
- Gitler, A.D. et al. (2004) Developmental Cell 7:107.
- Zhang, Y. et al. (2009) Dev. Biol. 325:82.
- Chauvet, S. et al. (2007) Neuron 56:807.
- Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
- Choi, Y.I. et al. (2008) Immunity 29:888.
- Toyofuku, T. et al. (2007) EMBO J. 26:1373.
- Roodink, I. et al. (2008) Am. J. Pathol. 173:1873.
Alternate Names
PLXND1
Gene Symbol
PLXND1
UniProt
Product Documents for Human Plexin D1 Antibody
Product Specific Notices for Human Plexin D1 Antibody
For research use only
Customer Reviews for Human Plexin D1 Antibody (2)
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 12/06/2019Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
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Application: ELISASample Tested: Serum and PlasmaSpecies: Human and MouseVerified Customer | Posted 11/08/2018
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