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Human Plasminogen/Plasmin Antibody

Catalog # MAB2596 | R&D Systems, Inc. a Bio-Techne Brand
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MAB2596
MAB2596-SP

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 270412

Product Summary for Human Plasminogen/Plasmin Antibody

Immunogen

Human plasma-derived Plasminogen

Specificity

Detects human Plasminogen and Plasmin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Plasminogen/Plasmin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human Plasminogen (Catalog # 1939-SE) under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB2596 in the following applications:

Published Applications

Read 3 publications using MAB2596 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plasminogen

Plasminogen (PLG) is the precursor of Plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion (1, 2). Synthesized in the kidney, Plasminogen is found in plasma and many extracellular fluids. Activated by u- or t-Plasminogen Activator, the single‑chain Plasminogen (aa 20-810) is converted to Plasmin, which consists of disulfide bond‑linked heavy chain A (aa 20-580) and light chain B (aa 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named Angiostatin, an angiogenesis inhibitor (3, 4).

References

  1. Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
  2. Forsgren, M. et al. (1987) FEBS Lett. 213:254.
  3. O’Reilly, M.S. et al. (1994) Cell 79:315.
  4. Sim, B.K. et al. (1997) Cancer Res. 57:1329.

Alternate Names

Plg

Entrez Gene IDs

5340 (Human); 18815 (Mouse); 85253 (Rat)

Gene Symbol

PLG

Product Documents for Human Plasminogen/Plasmin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Plasminogen/Plasmin Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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