Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 gamma Monoclonal Antibody (Catalog # MAB2999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PI 3-Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PI 3-Kinase p110 gamma Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p110 gamma

Class I phosphoinositide 3-kinases (PI 3-Kinases) are dual-specific lipid and protein kinases involved in numerous intracellular signaling pathways. Class IA PI 3‑Kinases (p110 alpha, beta and delta) are typically activated through receptor tyrosine kinase signaling, whereas p110 gamma, the single member of class IB, is activated by seven‑transmembrane G‑protein‑coupled receptors (GPCRs). While p110 alpha and beta are ubiquitiously expressed, expression of p110 gamma is mainly restricted to cells of the hematopoietic system.

Long Name

Protein Tyrosine Phosphatase 1G

Alternate Names

p120-PI3K, PI 3Kinase p110 gamma, PI3CG, PI3K-gamma, PIK3CG

Entrez Gene IDs

5294 (Human); 30955 (Mouse); 298947 (Rat)

Gene Symbol

PIK3CG

UniProt

P48736

Additional PI 3-Kinase p110 gamma Products

Product Documents for Human PI 3-Kinase p110 gamma Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PI 3-Kinase p110 gamma Antibody

For research use only

Citations
Reviews

Human PI 3-Kinase p110 gamma Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2999

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all PI 3-Kinase p110 gamma Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PI 3-Kinase p110 gamma Antibody

Detection of Human PI 3-Kinase p110 gamma by Western Blot.