Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110d Monoclonal Antibody (Catalog # MAB2687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 delta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human PI 3-Kinase p110 delta by Western Blot

Inhibition of PI3K delta and PI3K gamma subunits in MM proliferation. (a) Western blot analysis of PI3K delta and PI3K gamma subunits in MM cell lines and MM primary cells. Blots were probed with beta-actin to show sample loading. (b) MM primary cells were treated with idelalisib (1 μm), CZC24832 (1 μm) and duvelisib (1 μm) for 72 h and then assessed for cell viability using CellTiter Glo. (c) MM primary cells were treated with idelalisib (1 μm), CZC24832 (1 μm) and duvelisib (1 μm) for 72 h and then assessed for apoptosis by PI/Annexin V staining (n=4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/bcj201716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human PI 3-Kinase p110 delta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 4 reviews rated 4.3 using MAB2687 in the following applications:

ELISA (1 Review)
Microarray (1 Review)
Microarrays (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p110 delta

Class IA phosphoinositide 3-kinases (PI 3-kinases) are a family of p85/p110 heterodimeric lipid kinases that function as signal transducers downstream of growth factor receptors, mainly receptor tyrosine kinases. Mammals express three class IA catalytic subunits: p110 alpha, p110 beta, and p110 delta. The p110 delta subunit is expressed predominantly in leukocytes, indicating that it may play a role in immune signaling.

Long Name

Protein Tyrosine Phosphatase 1D

Alternate Names

PI 3Kinase p110 delta, PIK3CD

Entrez Gene IDs

5293 (Human)

Gene Symbol

PIK3CD

UniProt

O00329

Additional PI 3-Kinase p110 delta Products

Product Documents for Human PI 3-Kinase p110 delta Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human PI 3-Kinase p110 delta Antibody

For research use only

Citations
Reviews

Human PI 3-Kinase p110 delta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2687

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PI 3-Kinase p110 delta Antibody

Detection of Human PI 3-Kinase p110 delta by Western Blot.